郝阳毅;何雅琴;刘高坤;吕佳颖;孙晓格;马广亮;赵宏;王雅晶;李胜利;

• 1:中国农业大学动物科学技术学院动物营养国家重点实验室北京市生鲜乳质量安全工程技术研究中心
• 2:兰考中科华构生物科技有限公司

摘要(Abstract)：

为探究不同留茬高度对杂交构树产量、营养成分、青贮发酵品质及体外发酵的影响,本试验以生长高度为1.6 m的杂交构树为研究对象,分别设置20、35和50 cm 3个不同留茬高度进行收割(每个处理4个重复),并对其进行产量测定和营养成分分析。对不同留茬高度构树进行罐储,密度>1 000 kg/m~3,室温保存45 d,开罐之后再次进行营养成分分析,并对其青贮发酵品质进行测定,最后进行体外发酵试验。结果表明:当留茬高度从20 cm增加到35 cm,构树中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和总磷含量显著下降(P<0.05),粗蛋白质(CP)、钙(Ga)和总单宁含量显著上升(P<0.05),而构树产量、粗脂肪(EE)和粗灰分(Ash)含量无显著变化(P>0.05)。留茬高度从35 cm增加到50 cm,构树产量和NDF含量显著下降(P<0.05),EE、Ash和总单宁含量显著上升(P<0.05),而CP、ADF和总磷含量无显著变化(P>0.05)。干物质、木质素含量和缓冲能值不受留茬高度的影响,各组之间无显著差异(P>0.05)。不同留茬高度对构树青贮发酵品质影响很小,青贮pH、氨态氮(NH3-N)、乳酸、丙酸和丁酸含量在各组之间差异不显著(P>0.05)。但是留茬高度为50 cm的构树青贮中乙酸含量显著高于其他2组(P<0.05)。与鲜样相比,留茬高度为50 cm的构树青贮NDF含量显著降低(P<0.05)。不同留茬高度对构树青贮体外发酵干物质降解率(IVDMD)、产气量(GP)及发酵液NH3-N、微生物蛋白(MCP)含量没有显著影响(P>0.05)。但是相比于50 cm的留茬高度,35 cm留茬高度构树青贮能够显著增加发酵液中乙酸、丙酸、丁酸和总挥发性脂肪酸的含量(P<0.05)。综合考虑,构树最佳留茬高度为35 cm。

关键词(KeyWords)： 杂交构树;留茬高度;营养成分;青贮发酵;体外发酵

Abstract:

Keywords:

基金项目(Foundation): 现代农业（奶牛）产业技术体系建设专项（CARS-36）;; 宁夏回族自治区重点研发计划（2018BBF33006)

作者(Author): 郝阳毅;何雅琴;刘高坤;吕佳颖;孙晓格;马广亮;赵宏;王雅晶;李胜利;

Email:

DOI:

参考文献(References)：

• [1] PE?AILILLO J,OLIVARES G,MONCADA X,et al.Sex distribution of paper mulberry(Broussonetia papyrifera)in the pacific[J].PLoS One,2016,11(8):e0161148.
• [2]王子成，沈世华．航天杂交构树的特性与效益[J]．国土绿化，2013(5):47-48.
• [3] TANG F,CHEN N Z,ZHAO M L,et al.Identification and functional divergence analysis of WOX gene family in paper mulberry[J].International Journal of Molecular Sciences,2017,18(8):1782.
• [4] SI B W,TAO H,ZHANG X L,et al.Effect of Broussonetia papyrifera L.(paper mulberry)silage on dry matter intake,milk composition,antioxidant capacity and milk fatty acid profile in dairy cows[J]. AsianAustralasian Journal of Animal Sciences,2018,31(8):1259-1266.
• [5] KORNFELT L F,WEISBJERG M R,N?RGAARD P.Effect of harvest time and physical form of alfalfa silage on chewing time and particle size distribution in boli,rumen content and faeces[J].Journal of Animal Science,2013,7(2):232-244.
• [6] GETACHEW G,LACA E A,PUTNAM D H,et al.The impact of lignin downregulation on alfalfa yield,chemical composition,and in vitro gas production[J].Journal of Science Food Agriculture,2018,98(11):4205-4215.
• [7] LYNCH J P,BAAH J,BEAUCHEMIN K A.Conservation,fiber digestibility,and nutritive value of corn harvested at 2 cutting heights and ensiled with fibrolytic enzymes,either alone or with a ferulic acid esteraseproducing inoculant[J]. Journal of Dairy Science,2015,98(2):1214-1224.
• [8] NEYLON J M,KUNG L Jr.Effects of cutting height and maturity on the nutritive value of corn silage for lactating cows[J].Journal of Dairy Science,2003,86(6):2163-2169.
• [9] MANNERKORPI P,BRANDT M. Feeding value of whole-crop wheat silage for ruminants related to stage of maturity and cutting height[J]. Archiv für Tierernaehrung,1993,45(1):89-100.
• [10]黄彩变，曾凡江，雷加强．留茬高度对骆驼刺生长发育和产草量的影响[J]．草地学报，2011,19(6):948-953.
• [11]张国君，李云，孙宇涵，等．四倍体刺槐生物质产量影响因素的研究[C]//第八届中国林业青年学术年会论文集．哈尔滨：中国林学会，黑龙江省林学会，东北林业大学，2008:212-216.
• [12]宿爱芝．辣木栽培技术与生物量关系的研究[D]．硕士学位论文．北京：中国林业科学研究院，2012:26-
• [13] 3沈2．世华．杂交构树的“长相”和“性情”[J]．生命世界，2018(10):6-7.
• [14] BAUR F J,ENSMINGER L G.The association of official analytical chemists(AOAC)[J].Journal of the American Oil Chemists’Society,1977,54(4):171-172.
• [15] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597.
• [16] PLAYNE M J,MCDONALD P.The buffering constituents of herbage[J]. Journal of the Science of Food and Agriculture,2010,17(6):264-268.
• [17]赵微．不同生长期含单宁饲草单宁含量变化及其对瘤胃体外发酵的影响[D]硕士学位论文．延吉：延边大学，2015:9-10.
• [18] CHEN L,GUO G,YU C Q,et al. The effects of replacement of whole-plant corn with oat and common vetch on the fermentation quality,chemical composition and aerobic stability of total mixed ration silage in Tibet[J].Animal Science Journal,2015,86(1):69-76.
• [19] YUAN X J,WEN A Y,WANG J,et al.Effects of four short-chain fatty acids or salts on fermentation characteristics and aerobic stability of alfalfa(Medicago sativa L.)silage[J].Journal of the Science of Food and Agriculture,2018,98(1):328-335.
• [20] BAI S,CAO Z J,CAO B B,et al.Effects of different forage combinations in total mixed rations on in vitro gas production kinetics,ruminal and milk fatty acid profiles of lactating cows[J].Animal Science Journal,2018,89(9):1261-1270.
• [21] ZHANG D F,YANG H J. In vitro ruminal methanogenesis of a hay-rich substrate in response to different combination supplements of nitrocompounds;pyromellitic diimide and 2-bromoethanesulphonate[J].Animal Feed Science and Technology,2011,163(1):20-32.
• [22] MAKKAR H P,SHARMA O P,DAWRA R K,et al.Simple determination of microbial protein in rumen liquor[J]. Journal of Dairy Science,1982,65(11):2170-2173.
• [23]?RSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92(2):499-503.
• [24] LIM H,LEE K H,LEE K H,et al.Biomass expansion factors and allometric equations in an age sequence for Japanese cedar(Cryptomeria japonica)in southern Korea[J]. Journal of Forest Research,2013,18(4):316-322.
• [25] PEICHL M,ARAIN M A. Allometry and partitioning of above-and belowground tree biomass in an age-sequence of white pine forests[J]. Forest Ecology and Management,2007,253(1/2/3):68-80.
• [26]刘玉，林萌萌，郑爱华，等．杂交构树不同高度及不同部位的营养价值比较[J]．畜牧兽医杂志，2018,37(6):77-78,81.
• [27]刘春龙，李忠秋，孙海霞，等．影响青贮饲料品质的因素[J]．中国牛业科学，2006,32(5):63-66.
• [28] LYNCH J P,O’KIELY P,DOYLE E M.Yield,quality and ensilage characteristics of whole-crop maize and of the cob and stover components:harvest date and hybrid effects[J]. Grass and Forage Science,2012,67(4):472-487.
• [29]司丙文，徐文财，张小利，等．不同添加剂对杂交构树青贮发酵品质的影响[J]．动物营养学报，2018,30(11):4670-4675.
• [30]王学凯．添加乳酸菌和蔗糖对几种木本饲料青贮蛋白组分的影响[D]．硕士学位论文．北京：中国农业大学，2018:11-13.
• [31] KUNG L,Jr,SHAVER R D,GRANT R J,et al.Silage review:interpretation of chemical,microbial,and organoleptic components of silages[J]. Journal of Dairy Science,2018,101(5):4020-4033.
• [32] YANG J S,TAN H S,CAI Y M. Characteristics of lactic acid bacteria isolates and their effect on silage fermentation of fruit residues[J].Journal of Dairy Science,2016,99(7):5325-5334.
• [33] SILVA V P,PEREIRA O G,LEANDRO E S,et al.Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions[J].Journal of Dairy Science,2016,99(3):1895-1902.
• [34] MAHANNA B.Proper management assures high-quality silage,grains[J].Feedstuffs,1994,66(3):12-15.
• [35]张颖超．优良乳酸菌筛选及其在典型木本饲料青贮中的作用机制研究[D]．博士学位论文．北京：中国农业大学，2019:97-99.
• [36] JAWORSKI N W,L?RKE H N,KNUDSEN K E B,et al.Carbohydrate composition and in vitro digestibility of dry matter and nonstarch polysaccharides in corn,sorghum,and wheat and coproducts from these grains[J].Journal of Animal Science,2015,93(3):1103-1113.
• [37] KLJAK K,PINO F,HEINRICHS A J.Effect of forage to concentrate ratio with sorghum silage as a source of forage on rumen fermentation,N balance,and purine derivative excretion in limit-fed dairy heifers[J].Journal of Dairy Science,2017,100(1):213-223.
• [38] ASSOUMANI M B,VEDEAU F,JACQUOT L,et al.Refinement of an enzymatic method for estimating the theoretical degradability of proteins in feedstuffs for ruminants[J].Animal Feed Science and Technology,1992,39(3/4):357-368.
• [39] MATRAS J,BARTLE S J,PRESTON R L. Nitrogen utilization in growing lambs:effects of grain(starch)and protein sources with various rates of ruminal degradation[J].Journal of Animal Science,1991,69(1):339-347.
• [40] NSAHLAI I V,SIAW D,OSUJI P O. The relationships between gas production and chemical composition of 23 browses of the genus Sesbania[J].Journal of the Science of Food and Agriculture,1994,65(1):13-20.
• [41] CORNOU C,STORM I M L D,HINDRICHSEN I K,et al.A ring test of a wireless in vitro gas production system[J].Animal Production Science,2013,53(6):585-592.
• [42] GETACHEW G,ROBINSON P H,DEPETERS E J,et al.Relationships between chemical composition,dry matter degradation and in vitro gas production of several ruminant feeds[J]. Animal Feed Science and Technology,2004,111(1/2/3/4):57-71.
• [43]左鑫，陈哲，谢强，等．不同产地构树叶粉和构树枝叶粉营养成分及其鹅代谢能的测定[J]．动物营养学报，2018,30(7):2823-2830.