章世元;俞路;王雅倩;孙怀昌;王志跃;王捍东;周联高;严桂芹;张莹;林显华;汪益峰;闫韩韩;

• 1:扬州大学动物科学与技术学院
• 2:扬州大学兽医学院
• 3:山东省胶南市海青动物防疫监督站

摘要(Abstract)：

试验选用56周龄新扬州鸡产蛋鸡150只,随机分为3个处理,每个处理5个重复,各重复10只。研究经腿肌注射0、200和400μg鸡甲状旁腺素基因质粒(pCEP4-PTH)对蛋鸡生产性能、蛋壳质量和血液部分激素的影响。试验结果表明,pCEP4-PTH基因质粒可改善蛋鸡生产性能;200μg注射量可显著改善蛋壳强度(P<0.05),400μg注射量对蛋壳质量影响不显著;pCEP4-PTH基因质粒可在蛋鸡体内良好表达,甲状旁腺素(PTH)水平均显著升高(P<0.05),且注射400μg时达到极显著水平(P<0.01),注射400μg pCEP4-PTH基因质粒具有促进内源降钙素(CT)和雌激素(E2)分泌的作用(P<0.05),因而不利于其生物学效应的发挥,两试验组骨钙素(BGP)与对照组差异不显著,提示生产性能和蛋壳质量的改善未对骨骼造成不良影响。pCEP4-PTH基因质粒可改善蛋鸡生产性能和蛋壳质量,且不影响骨骼质量。

关键词(KeyWords)： pCEP4-PTH基因质粒;蛋鸡;生产性能;蛋壳质量;激素

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 章世元;俞路;王雅倩;孙怀昌;王志跃;王捍东;周联高;严桂芹;张莹;林显华;汪益峰;闫韩韩;

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