铁对泌乳期小鼠乳腺中乳铁蛋白基因mRNA水平的影响Effects of Iron on LF Gene Expression of Mice Mammary Gland
马文霞;乔爱君;潘晓亮;钱克栋;石文艳;
摘要(Abstract)：
探讨铁对小鼠乳腺中乳铁蛋白(lactoferrin,LF)mRNA基因表达的影响。选取雌性昆明白小鼠40只,随机分成5组,按实验动物标准饲养,各处理组添加不同水平和来源的铁:硫酸亚铁(Fe0mg/kg)、硫酸亚铁(Fe60mg/kg)、硫酸亚铁(Fe120mg/kg)、甘氨酸铁(Fe120mg/kg)、甘氨酸铁+硫酸亚铁(Fe120mg/kg)。配种、产仔,于哺乳21d后处死取样,用相对定量RT-PCR方法,以β-actin作内标,测乳腺LFmRNA丰度。试验表明,不同来源和剂量的铁可不同程度影响LFmRNA的表达,甘氨酸铁(Fe120mg/kg)组较对照组差异达极显著水平(P<0.01)。因此,甘氨酸铁可显著提高乳腺中LFmRNA的表达量,作为有效铁补充剂的同时可能促进乳汁中LF的含量。
关键词(KeyWords)： 铁;小鼠;乳腺;LF;基因表达
基金项目(Foundation):
作者(Author): 马文霞;乔爱君;潘晓亮;钱克栋;石文艳;
Email:
DOI:
参考文献(References)：
- [1]Legrand D,Elass E,Carpentier M,Mazurier J.Lact-oferrin:a modulator of i mmune and inflammatory responses.Cellular and Molecular Life Science,2005,1-11.
- [2]Tat'yana G,Kanyshkova,Svetlana E,Babina,Dmitry V.Semenov,Natal'ya Isaeva,Alexander V,Vlassov,Kirill N,Neustroev,Anna A,Kul'minskaya,Valentina N,Buneva,Georgy A,Nevinsky.Mutiple enzymic ac-tivities of human milk lactoferrin.European Journal of Biochemistry,2003:270:3353-3361.
- [3]Jillian Cornish,Karen E Callon,Dorit Naot,Kate P Pal mano,Tatjana Banovic,Usha Bava,Maureen Watson,Jian-Ming Lin,P C Tong,Qi Chen,Vincent A Chan,Helen E Reid,Nick Fazzalari,Heather M Baker,Edward N Baker,Neill W Haggarty,Andrew B Grey,Ian R Reid.Lactoferrinis a potent regulator of bone cell activity and increase bone for mation in vivo.Endocrinology,2004,145(9):4366-4374.
- [4]Brock J H.The physioloty of lactoferrin.Biochem.Biochemistry and Cell Biology,2002,80:1-6.
- [5]Wang Y,Tu Y,Han F,Xu Z,Wang J.Developmen-tal gene expression of lactoferrin and effect of dieta-ry iorn on gene regulation of lactoferrin in mouse mammary gland.Journal of Dairy Science,2005,88:2065-2071.
- [6]杨萍.简明实验动物(第一版).上海:复旦大学出版社,2003.
- [7]钟品仁.哺乳类实验动物(第一版).北京:人民卫生出版社,1983.
- [8]Babina S E,Kanyshkova T G,Buneva V N,Nevinsky G A.Lactoferrin is the major deoxyribonuclease of human milk.Bichemistry,2004,69(9):1006-1015.