中性植酸酶在豆粕型饲料中替代磷酸二氢钙对花鲈生长及磷代谢的影响Effect of Replacement Calcium Phosphate Monobasic with Neutral Phytase on Growth and Phosphorous Metabolism of Japanese Sea Bass (Lateolabrax Japonicus)
罗琳;吴秀峰;薛敏;曹海宁;柏文东;姚斌;
摘要(Abstract):
本文旨在研究豆粕型饲料中用中性植酸酶替代不同水平的磷酸二氢钙,对花鲈(Lateolabrax japonicus)生长和磷代谢及利用的影响。以(2.52±0.01)g的花鲈为试验对象,用中性植酸酶分别替代试验用的豆粕型饲料中60%(PE6)、80%(PE8)和100%(PET)的磷酸二氢钙,每个替代水平饲料中分别设置1000FTU/kg(PE610,PE810,PET10)和1500FTU/kg(PE615,PE815,PET15)2个梯度。8周的养殖试验结果显示,在豆粕型饲料中用中性植酸酶替代磷酸二氢钙后,对各试验组花鲈的生长、鱼体成分和蛋白质消化率均无显著影响(P>0.05);但PE615和PE815组的干物质消化率显著高于对照组(P<0.05),PE815组的脂肪消化率显著高于PE610、PET10和PET15组(P<0.05),而PE615和PE815的总磷消化率显著高于对照组、PE610组和PET15组的(P<0.05)。各试验组的总磷摄入量、表观磷的摄入量、粪磷和非粪磷的排出量均随试验料中磷酸二氢钙添加量的减少而显著降低(P<0.05),而除PE610和PE815以外的各试验组的总磷储积率却显著高于对照组(P<0.05)。根据本试验的结果,在含有30.5%豆粕的花鲈饲料中添加一定量的中性植酸酶同时减少磷酸二氢钙的添加量后,仍能显著提高饲料中磷的消化和利用(P<0.05)。中性植酸酶的添加量以1000FTU/kg饲料为宜,其作用相当于12g/kg的磷酸二氢钙。
关键词(KeyWords): 花鲈(Lateolabrax japronicus);中性植酸酶;磷酸二氢钙
基金项目(Foundation): 北京市科委资助项目(H012010330113)
作者(Author): 罗琳;吴秀峰;薛敏;曹海宁;柏文东;姚斌;
Email:
DOI:
参考文献(References):
- [1]NRC.Nutrient Requirements of Fish.National A-cademy Press,Washington,D.C.,1993.
- [2]Ketola H G,Harland B F.Influence of phosphorus in rainbow trout diets on phosphorus discharges in effluent water.Transactions of the American Fish-eries Society,1993,122:1120-1126.
- [3]Cho C Y,Bureau D P.Reduction of waste output fromsal monid aquaculture through feeds and feed-ing.The Progressive Fish-Culturist,1997,59:155-160.
- [4]曾虹.植酸酶的研究进展及其在鱼饲料中的应用前景.上海水产大学学报,1998,7(8):39-43.
- [5]贺建华.植酸磷和植酸酶研究进展.动物营养学报,2005,17(1):1-6.
- [6]曾虹,姚斌,任泽林.中性植酸酶NPHYA的酶学性质及其在鲤鱼饲料中的应用效果.水生生物学报,2002,26(增刊):67-74.
- [7]Markus R,Zoltan G,Ernst P.Effect of phosphorus intake on faecal and non-faecal phosphorus excre-tion in rainbow trout(Oncorhynchus mykiss)andthe consequences for comparative phosphorus avail-ability studies.Aquaculture,2000,188:383-398.
- [8]Satoh S,Porn-Ngam N,Takeuchi T,Watanabe T.Influence of dietary phosphorus levels of growth and mineral availability in rainbow trout.Fish Science,1996,62:483-487.
- [9]常青.花鲈对常用饲料原料中氨基酸和磷的利用率研究.海洋水产研究,2004,2:35-40.
- [10]Oliva-Teles A,Pereira J P,Gouveia A,Gomes,E.Utilization of diets supplemented with microbial phytase by seabass(Dicentrarchus labrax)juveniles.Aquatic Living Resources,1998,11(4):255-259.
- [11]Riche M,Brown P B.Availability of phosphorus for m feedstuffs fed to rainbour trout,Oncorhyn-chus mykiss.Aquaculture,1996,142:269-282.
- [12]Soares J H.Efficacy of Phytase on Phosphorus Uti-lization.Proceeding of Maryland Nutrition Confer-ence,1994,76.
- [13]Jackson L S,Li M H,Robinson E H.Use of micro-bial phytase in Channel catfish Ictalurus punctatus diets to i mprove utilization of phytase phosphorus.Journal of World Aquaculture Society,1996,27:309-313.
- [14]Cheng ZJ,Hardy R W.Effects of microbial phytase supplementation and dosage on apparent digestibility coefficients of nutrients and dry matter in soybean product-based diets for rainbowtrout(Oncorhyn-chus mykiss).Aquaculture Nutrition,2002,8:271-277.
- [15]Cheng Z J,Hardy R W.Effects of extrusion and expelling processing,and microbial phytase supple-mentation on apparent digestibility coefficients of nutrients in full-fat soybeans for rainbow trout(Oncorhynchus mykiss).Aquaculture,2003,218:501-514.
- [16]Cheng ZJ,Hardy R W.Apparent digestibility coef-ficients of nutrients arei mproved by phytase supple-mentationin corn distiller’s dried grain with soluble for rainbowtrout(Oneorhynehus mykiss).Journal of Ani mal Science,2002,80(Suppl1):345-346.
- [17]宋金彩,单安山.植酸酶对蛋白质和氨基酸利用率的影响.中国饲料,2000,10:14-15.